EK981EGA

Easy Go!™ Human/Mouse/Rat TGF-β1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

X

¥2,190.00¥3,650.00

  • 分子靶点:TGFB1, TGB-β1, TGFB
  • 种属:人 (Human); 小鼠 (Mouse); 大鼠(Rat)
  • 样本类型:血清,血浆
  • 检测样本体积:
    • 人血清: 活化样本80μL + 720μL Assay Buffer
    • 小鼠/大鼠血清:活化样本20μL + 480μL Assay Buffer
    • 人/大鼠/小鼠血浆:活化样本80μL + 80μL Assay Buffer
  • 灵敏度:10.76 pg/mL
  • 检测范围:15.625 pg/mL - 1000 pg/mL
  • 回收率:75% - 116%

在售SKU:70-EK981EGA-48, 70-EK981EGA-96, EK981EGA


描述

商品名

Easy Go!™ Human/Mouse/Rat TGF-β1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:5.8% - 9.1%;板间变异系数:4.3% - 6.9%

样本类型

血清,血浆

检测样本体积
  • 人血清: 活化样本80μL + 720μL Assay Buffer
  • 小鼠/大鼠血清:活化样本20μL + 480μL Assay Buffer
  • 人/大鼠/小鼠血浆:活化样本80μL + 80μL Assay Buffer
灵敏度

10.76 pg/mL

检测范围

15.625 pg/mL - 1000 pg/mL

回收率

75% - 116%

平均回收率

0.94

板式

96孔板,可拆

保存

试剂盒未拆开,2 - 8℃保存。已拆开,标准品-20℃保存,其它2 - 8℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 10×检测缓冲液
  • 显色底物
  • 终止液
  • 20×洗液
  • HCl
  • NaOH

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入生物素标记的检测抗体微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450 nm 波长 (参考波长 5630 nm) 测定吸光度值。

EasyGo!™ Human/Mouse/Rat TGF-β1 ELISA Kit EasyGo!™ 人/小鼠/大鼠转化生长因子β1 (TGF-β1) 一步法酶联免疫吸附测定试剂盒 - 标准曲线
标准曲线

分子信息

概述

TGFB1 分子靶点信息概述

  • 分子名:TGFB1, transforming growth factor beta 1
  • 基因家族:Transforming growth factor beta family
  • 别名:CED; TGFbeta
  • 曾用名:TGFB; DPD1
  • 全称:Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1 分子靶点综述

转化生长因子β1 (TGF-β1)是转化生长因子β超家族的多肽成员,具有多种细胞学功能,包括调控细胞生长、增殖、分化和凋亡。TGF-β与TGFA协同诱导转化。它也能作为负向自分泌生长因子。TGF-β活化和信号传导的失调可能会引起凋亡。很多细胞可合成TGF-β,且几乎都有特异性的受体。TGF-β1、TGF-β2和TGF-β3都通过相同的受体信号通路发挥功能。TGF-β1对调控免疫系统具有重要作用,对不同类型的细胞或不同发育阶段的细胞具有不同的活性。大部分免疫细胞 (或白细胞) 分泌TGF-β1。TGF-β1与癌症、自身免疫疾病、肝脏疾病、肾脏疾病、糖尿病、心血管疾病、哮喘、慢性阻塞性肺疾病 (COPD)和囊肿性纤维化 (CF) 等相关。

人 Human TGFB1 分子靶点信息

  • 分子名:TGFB1, transforming growth factor beta 1
  • 别称:
    • CED
    • DPD1
    • IBDIMDE
    • LAP
    • latency-associated peptide
    • prepro-transforming growth factor beta-1
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta1
    • TGFB
    • TGFbeta
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor beta1
  • 基因序列:NCBI_Gene: 7040
  • 蛋白序列:UniProtKB: P01137

人 Human TGFB1靶点分子功能(预测)

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet's disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).

操作步骤


ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


  1. Glypican-3-targeted macrophages delivering drug-loaded exosomes offer efficient cytotherapy in mouse models of solid tumours 

  2. A nanotheranostics with hypoxia-switchable fluorescence and photothermal effect for hypoxia imaging-guided immunosuppressive tumor microenvironment modulation 

  3. Serum amyloid A contributes to radiation-induced lung injury by activating macrophages through FPR2/Rac1/NF-κB pathway 

  4. Localized light-triggered release macrophage cytopharmaceuticals containing O-nitrobenzyl group for enhanced solid tumor cell-chemotherapy 

  5. Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 

  6. siTGF-β1 and pirfenidone contained Ionizable-Liposomal nanodrug for enhanced treatment of Idiopathic pulmonary fibrosis 

  7. Sanziguben Polysaccharides Attenuate Renal Epithelial-Mesenchymal Transition in Diabetic Nephropathy through Nrf2-Mediated Regulation of TGF-β1/Smad7 Signaling Pathway 

  8. miR-455/GREM1 axis promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing M2 macrophage polarization 

  9. CD38 and extracellular NAD+ regulate the development and maintenance of Hp vaccine‐induced CD4+ TRM in the gastric epithelium 

  10. Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming 

评价

目前还没有评价

成为第一个“Easy Go!™ Human/Mouse/Rat TGF-β1 ELISA Kit 检测试剂盒(酶联免疫吸附法)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注