EK106EGA

EasyGo!™ Human IL-6 One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

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¥2,190.00 – ¥3,650.00

  • 分子靶点：IL6, IL-6, IFNB2, HGF
  • 种属：人 (Human)
  • 样本类型：血清，血浆
  • 检测样本体积：20 μL
  • 灵敏度：1.05 pg/mL
  • 检测范围：1.56 pg/mL - 100 pg/mL
  • 回收率：76% - 125%

在售SKU:70-EK106EGA-48, 70-EK106EGA-96, 人白细胞介素6, 白细胞介素6, 白细胞介素, 人, 白介素, 6, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

描述

商品名

EasyGo!™ Human IL-6 One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法

一步法
精密度

板内变异系数：7.1% - 9.5%；板间变异系数：2.8% - 9.2%
样本类型

血清，血浆
检测样本体积

20 μL
灵敏度

1.05 pg/mL
检测范围

1.56 pg/mL - 100 pg/mL
回收率

76% - 125%
平均回收率

1.00
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™ Human IL-6 One-Step ELISA Kit EasyGo!™ 人白细胞介素6 (IL-6) 一步法酶联免疫吸附测定试剂盒- 标准曲线
标准曲线

分子信息

概述人 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名：IL6, interleukin 6
  • 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名：IL-6; BSF2; HGF; HSF
  • 曾用名：IFNB2
  • 全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

人 Human IL6 分子靶点信息

  • 分子名：IL6, interleukin 6
  • 别称：
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • 基因序列：NCBI_Gene: 3569
  • 蛋白序列：UniProtKB: P05231

人 Human IL6靶点分子功能（预测）

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

操作步骤

检测步骤简略图示


ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

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  11. Effects of silencing NLRP3 gene on proliferation of psoriasis-like HaCaT cells and expressions of cytokines 

  12. PM2.5 exposure upregulates pro-inflammatory protein expression in human microglial cells via oxidant stress and TLR4/NF-κB pathway 

  13. Structure and biological activity in vitro of Flagellin and its mutants from Escherichia coli Nissle 1917 

  14. Circ_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis 

  15. Klotho exerts protection in chronic kidney disease associated with regulating inflammatory response and lipid metabolism 

  16. Adipocyte enhancer binding protein 1 knockdown alleviates osteoarthritis through inhibiting NF-κB signaling pathway-mediated inflammation and extracellular matrix degradation 

  17. Targeting osteopontin alleviates endometriosis and inflammation by inhibiting the RhoA/ROS axis and achieves non-invasive in vitro detection via menstrual blood 

  18. Poly(rC)-binding protein 1 alleviates neurotoxicity in 6-OHDA-induced SH-SY5Y cells and modulates glial cells in neuroinflammation 

  19. High Expression of miR-6785-5p in the Serum Exosomes of Psoriasis Patients Alleviates Psoriasis-Like Skin Damage by Interfering with the MNK2/p-eIF4E Axis in Keratinocytes 

  20. Comparison of volume-controlled ventilation, pressure-controlled ventilation and pressure-controlled ventilation-volume guaranteed in infants and young children in the prone position: A prospective randomized study 

  21. Surface Modification of Zn–Cu Alloy with Heparin Nanoparticles for Urinary Implant Applications 

  22. CircACTR2 attenuated the effects of tetramethylpyrazine on human kidney cell injury 

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  24. Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier 

  25. Enhancing immune regulation in vitro: the synergistic impact of 3′-sialyllactose and osteopontin in a nutrient blend following influenza virus infection 

  26. FSTL1 Accelerates Nucleus Pulposus Cell Senescence and Intervertebral Disc Degeneration Through TLR4/NF-κB Pathway 

  27. Analysis of intestinal flora in elderly Uygur patients with sarcopenia 

  28. LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 

  29. Novel Applications of Yinhua Miyanling Tablets in Ulcerative Colitis Treatment Based on Metabolomics and Network Pharmacology 

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  34. (–)-Syringaresinol Attenuates Ulcerative Colitis By Improving Intestinal Epithelial Barrier Function And Inhibiting Inflammatory Responses 

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