EK8100
Cortisol Competitive ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,900.00 – ¥3,200.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:待定
- 种属:人 (Human); 小鼠 (Mouse); 大鼠 (Rat)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:66.43 pg/mL
- 检测范围:0.12 ng/mL - 15 ng/mL
- 回收率:80% - 130%
在售SKU:70-EK8100-48, 70-EK8100-96
描述
商品名 |
Cortisol Competitive ELISA Kit (皮质醇竞争法 ELISA试剂盒) |
---|---|
检测方法 |
竞争法 |
精密度 |
板内变异系数:4.3% - 4.6%;板间变异系数:6.4% - 7.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
66.43 pg/mL |
检测范围 |
0.12 ng/mL - 15 ng/mL |
回收率 |
80% - 130% |
平均回收率 |
1.18 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用固相竞争酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。皮质醇特异性的单克隆抗体加入至孔中,经过孵育,与固相抗体结合。洗涤后,加入辣根过氧化物酶标记的皮质醇和未标记的皮质醇或样本,竞争结合单克隆抗体上有限的结合位点。在洗涤去除未结合的物质后,加入显色底物,颜色反应的深浅与皮质醇的浓度成反比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本,但其它物种的皮质醇预计也可被检测。
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Effects of resveratrol on intestinal oxidative status and inflammation in heat-stressed rats 
- Total glycosides from stems of Cistanche tubulosa alleviate depression-like behaviors: bidirectional interaction of the phytochemicals and gut microbiota 
- Hydroxytyrosol ameliorates stress–induced liver injury through activating autophagy via HDAC1/2 inhibition 
- Antidepressant effects of esketamine via the BDNF/AKT/mTOR pathway in mice with postpartum depression and their offspring 
- Mental health, sleep quality, and hormonal circadian rhythms in pregnant women with threatened preterm labor: a prospective observational study 
- Plasma biomarkers and delirium in critically ill patients after cardiac surgery: A prospective observational cohort study 
Effects of resveratrol on intestinal oxidative status and inflammation in heat-stressed rats 
Total glycosides from stems of Cistanche tubulosa alleviate depression-like behaviors: bidirectional interaction of the phytochemicals and gut microbiota 
Hydroxytyrosol ameliorates stress–induced liver injury through activating autophagy via HDAC1/2 inhibition 
Antidepressant effects of esketamine via the BDNF/AKT/mTOR pathway in mice with postpartum depression and their offspring 
Mental health, sleep quality, and hormonal circadian rhythms in pregnant women with threatened preterm labor: a prospective observational study 
Plasma biomarkers and delirium in critically ill patients after cardiac surgery: A prospective observational cohort study 
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