AT107

Annexin V-APC/PI Apoptosis Kit (贴壁细胞专用)

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¥1,010.00 – ¥2,090.00

在售SKU:70-AT107-100, 70-AT107-60, 70-AT107-30, 凋亡, 细胞凋亡, 凋亡试剂盒, 细胞凋亡试剂盒, 凋亡检测, 细胞凋亡检测, 凋亡检测试剂盒, 细胞凋亡检测试剂盒, Annexin V, Annexin, Apoptosis, 贴壁, 贴壁细胞

描述

产品简介

Annexin V(或 Annexin A5)为胞内蛋白膜联蛋白家族成员，以钙依赖的方式与磷脂酰丝氨酸(PS)结合。PS存在于正常细胞浆膜的内层，但在凋亡早期，膜不对称性丧失，PS 易位至细胞表面。荧光标记的 Annexin V可与之特异性结合，表明该细胞为凋亡细胞。
PI(碘化丙啶)是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但可以透过凋亡晚期和坏死细胞的细胞膜而使细胞核染红。因此，将 Annexin V 与 PI 联合使用时，PI 则被排除在活细胞(Annexin V-/PI-)和早期凋亡细胞(Annexin V+/PI-)之外，而晚期凋亡细胞和坏死细胞同时被荧光标记的Annexin V 和 PI 结合染色呈现双阳性(Annexin V+/PI+)。
Accutase 是一种天然的酶，兼具蛋白水解酶和胶原酶的活性。但它比胰酶和胶原酶更有效，使用浓度更低，使之毒性更小、更温和。因此对细胞的损伤更小，可减少因消化处理带来的假阳性。即便长时间消化，也可保持 90%以上的细胞活力。适合于各种贴壁细胞、胚胎和神经元干细胞等较敏感的细胞的消化。
本试剂盒仅需 15 分钟即可染色样本，快速方便。提供阳性质控液，便于制备单染管，调节补偿。提供消化细胞更温和的 Accutase，减少假阳性。即便长时间消化，也可保持 90%以上的细胞活力。适合于各种贴壁细胞、胚胎和神经元干细胞等较敏感的细胞的消化。本试剂盒仅需 15 分钟即可染色样本，快速方便。提供阳性质控液，便于制备单染管，调节补偿。提供消化细胞更温和的 Accutase，减少假阳性。

产品特点

  • 操作简便，15分钟即可完成染色；
  • 分群清晰，结果稳定可靠；
  • 独家搭配阳性质控液，补偿调节不再困难；
  • 提供Accutase Enzyme，适用于贴壁细胞。

结果示例

试剂盒组分

专利｜一种检验凋亡试剂真假的专利

专利｜一种检验凋亡试剂真假的专利 

流式实验中不可或缺的试剂—辅助试剂

流式实验中不可或缺的试剂—辅助试剂 

新品发布 | 大鼠Foxp3流式抗体上市了，速来体验吧

新品发布 | 大鼠Foxp3流式抗体上市了，速来体验吧 

Accutase酶：凋亡检测最佳辅助

Accutase酶：凋亡检测最佳辅助 

论文分享 | 联科凋亡试剂助力巨噬细胞机理研究

论文分享 | 联科凋亡试剂助力巨噬细胞机理研究 

引用文献

文章目录[隐藏]


  1. Root Extract of Lindera aggregata (Sims) Kosterm. Modulates the Th17/Treg Balance to Attenuate DSS-Induced Colitis in Mice by IL-6/STAT3 Signaling Pathway 

  2. LincRNA-p21 knockdown reversed tumor-associated macrophages function by promoting MDM2 to antagonize* p53 activation and alleviate breast cancer development 

  3. Targeting hexokinase 2 increases the sensitivity of oxaliplatin by Twist1 in colorectal cancer 

  4. Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3-mediated N6-methyladenosine modification of ICAM2 mRNA in fibroblast-like synoviocytes 

  5. Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system 

  6. Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process 

  7. STRA6 exerts oncogenic role in gastric tumorigenesis by acting as a crucial target of miR-873 

  8. Preclinical efficacy and involvement of mTOR signaling in the mechanism of Orf virus against nasopharyngeal carcinoma cells 

  9. Novel isatin-based hybrids as potential anti-rheumatoid arthritis drug candidates: Synthesis and biological evaluation 

  10. Knockdown of DUXAP10 inhibits proliferation and promotes apoptosis in bladder cancer cells via PI3K/Akt/mTOR signaling pathway 

  11. The Aurora Kinase Inhibitor TAK901 Inhibits Glioblastoma Growth by Blocking SREBP1-Mediated Lipid Metabolism 

  12. TJ-M2010-5, a novel CNS drug candidate, attenuates acute cerebral ischemia-reperfusion injury through the MyD88/NF-κB and ERK pathway 

  13. Hyperhomocysteinemia exacerbates acute kidney injury via increased mitochondrial damage 

  14. The direct miR-874-3p-target FAM84A promotes tumor development in papillary thyroid cancer 

  15. CYP2E1 changes the biological function of gastric cancer cells via the PI3K/Akt/mTOR signaling pathway 

  16. Chlamydia trachomatis Plasmid Protein pORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival via MAPK/p38 Pathway 

  17. Long noncoding RNA FAM225A promotes the malignant progression of gastric cancer through the miR-326/PADI2 axis 

  18. Long non-coding RNA CCDC144NL-AS1 sponges miR-143-3p and regulates MAP3K7 by acting as a competing endogenous RNA in gastric cancer 

  19. Circular RNA CDR1as Alleviates Cisplatin-Based Chemoresistance by Suppressing MiR-1299 in Ovarian Cancer 

  20. Metabotropic glutamate receptor 3 is involved in B-cell-related tumor apoptosis 

  21. Effect of benzo[a]pyrene on proliferation and metastasis of oral squamous cell carcinoma cells: A transcriptome analysis based on RNA-seq 

  22. A Novel Age-Related Circular RNA Circ-ATXN2 Inhibits Proliferation, Promotes Cell Death and Adipogenesis in Rat Adipose Tissue-Derived Stromal Cells 

  23. Preliminary mechanism in fetal alloimmune thrombocytopenia associated with anti-HPA 15b antibodies 

  24. LGALS4 as a Prognostic Factor in Urothelial Carcinoma of Bladder Affects Cell Functions 

  25. Short-term Pharmacological Inhibition of MyD88 Homodimerization by a Novel Inhibitor Promotes Robust Allograft Tolerance in Mouse Cardiac and Skin Transplantation 

  26. Long noncoding RNA FER1L4 promotes the malignant processes of papillary thyroid cancer by targeting the miR-612/ Cadherin 4 axis 

  27. Albumin-based nanoparticles combined with photodynamic therapy enhance the antitumor activity of curcumin derivative C086 

  28. Reduced apurinic/apyrimidinic endonuclease activity enhances the antitumor activity of oxymatrine in lung cancer cells 

  29. STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer 

  30. The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers 

  31. Downregulation of miR-122 attenuates hypoxia/reoxygenation (H/R)-induced myocardial cell apoptosis by upregulating GATA-4 

  32. Metformin alleviates dexamethasone-induced apoptosis by regulating autophagy via AMPK/mTOR/p70S6K in osteoblasts 

  33. Interferon‑stimulated gene 15 promotes progression of endometrial carcinoma and weakens antitumor immune response 

  34. Targeting NAD metabolism regulates extracellular adenosine levels to improve the cytotoxicity of CD8+ effector T cells in the tumor microenvironment of gastric cancer 

  35. Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes 

  36. Secoisolariciresinol diglucoside-derived metabolite, enterolactone, attenuates atopic dermatitis by suppressing Th2 immune response 

  37. Reelin depletion alleviates multiple myeloma bone disease by promoting osteogenesis and inhibiting osteolysis 

  38. NUSAP1 promotes gastric cancer radioresistance by inhibiting ubiquitination of ANXA2 and is suppressed by miR-129-5p 

  39. Extracellular vesicles from alveolar macrophages harboring phagocytosed methicillin-resistant Staphylococcus aureus induce necroptosis 

  40. The cumulative antitumor effects of regorafenib and radiotherapy in hepatocellular carcinoma 

  41. Detoxification of DON-induced hepatotoxicity in mice by cold atmospheric plasma 

  42. BET Inhibitor JQ1 Selectively Reduce Tregs by Upregulating STAT3 and Suppressing PD-1 Expression in Patients with Multiple Myeloma 

  43. P. gingivalis in oral-prostate axis exacerbates benign prostatic hyperplasia via IL-6/IL-6R pathway 

  44. ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway 

  45. Preclinical efficacy of CBR-5884 against epithelial ovarian cancer cells by targeting the serine synthesis pathway 

  46. Polymorphism rs2327430 in TCF21 predicts the risk and prognosis of gastric cancer by affecting the binding between TFAP2A and TCF21 

  47. Vaporization phosphorization-mediated synthesis of phosphorus-doped TiO2 nanocomposites for combined photodynamic and photothermal therapy of renal cell carcinoma 

  48. PD-1 downregulation enhances CAR-T cell antitumor efficiency by preserving a cell memory phenotype and reducing exhaustion 

  49. Fucoidan suppresses proliferation and epithelial-mesenchymal transition process via Wnt/β-catenin signalling in hemangioma 

  50. WDR4 promotes HCC pathogenesis through N7-methylguanosine by regulating and interacting with METTL1 

  51. C/EBPα-p30 confers AML cell susceptibility to the terminal unfolded protein response and resistance to Venetoclax by activating DDIT3 transcription 

  52. Identification of COQ2 as a regulator of proliferation and lipid peroxidation through genome-scale CRISPR-Cas9 screening in myeloma cells 

  53. The combination of Tanshinone IIA and Astragaloside IV attenuates myocardial ischemia–reperfusion injury by inhibiting the STING pathway 

  54. Closed-loop of Catalyzed Lactate Oxidization Synergistically Sensitizes Apoptosis and Ferroptosis for Cancer Therapy 

  55. Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model 

  56. Absent in melanoma 2 attenuates proliferation and migration and promotes apoptosis of human colorectal cancer cells by activating P38MAPK signaling pathway 

  57. Synergistic anti-oxidant and anti-inflammatory effects of ceria/resatorvid co-decorated nanoparticles for acute lung injury therapy 

  58. Single-cell sequencing analysis related to sphingolipid metabolism guides immunotherapy and prognosis of skin cutaneous melanoma 

  59. Crosstalk of RNA methylation writers defines tumor microenvironment and alisertib resistance in breast cancer 

  60. Combination of irinotecan silicasome nanoparticles with radiation therapy sensitizes immunotherapy by modulating the activation of the cGAS/STING pathway for colorectal cancer 

  61. CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma 

  62. Identification of a natural PLA2 inhibitor from the marine fungus Aspergillus sp. c1 for MAFLD treatment that suppressed lipotoxicity by inhibiting the IRE-1α/XBP-1s axis and JNK signaling 

  63. m7G-related genes predict prognosis and affect the immune microenvironment and drug sensitivity in osteosarcoma 

  64. Long non-coding RNA PANDAR promoted radiation and cisplatin-induced DNA damage repair through ATR/CHK1 in NSCLC 

  65. NKD1 targeting PCM1 regulates the therapeutic effects of homoharringtonine on colorectal cancer 

  66. FUNDC1 modulates mitochondrial defects and pancreatic β-cell dysfunction under lipotoxicity 

  67. Targeting NRF2 uncovered an intrinsic susceptibility of acute myeloid leukemia cells to ferroptosis 

  68. Cartilage-targeting mRNA-lipid nanoparticles rescue perifocal apoptotic chondrocytes for integrative cartilage repair 

  69. Albumin-encapsulated HSP90-PROTAC BP3 nanoparticles not only retain protein degradation ability but also enhance the antitumour activity of BP3 in vivo 

  70. Overexpression of CASP1 triggers acute promyelocytic leukemia cell pyroptosis and differentiation 

  71. Spermatogenesis associated serine rich 2 like plays a prognostic factor and therapeutic target in acute myeloid leukemia by regulating the JAK2/STAT3/STAT5 axis 

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