eFluor?450 染料档案

近期发表文章：
Regulatory T-cell microRNA expression changes in children with acute Kawasaki disease

Fen-fen Ni … Jun Yang, Clinical & Experimental Immunology | 07/16/2014

... Whole blood samples were processed for analysis by first incubating with anti-human CD4-eFluor 450 and anti-human CD25-PerCP-Cy5.5 (eBioscience, USA) at 4°C for 30 min. ... and stained with anti-human Foxp3-APC (eBioscience), ...

P2X-selective purinergic antagonists are strong inhibitors of HIV-1 fusion during both cell-to-cell and cell-free infection

Talia H. Swartz, … Benjamin K. Chen, Journal of Virology | 07/16/2014

… purified by Ficoll-Hypaque density gradient centrifugation. MT4 (target) cells were dye- 166 labeled with 5 µM eFluor 450 (eBioscience) for 10 min at 37°C. …

Toll-like receptor 9 signaling acts on multiple elements of the germinal center to enhance antibody responses

Derek C. Rookhuizen and Anthony L. DeFranco, PNAS, Jul 2014; 10.1073/pnas.1323985111.

...and IgD-APC and GL7-APC (eBioscience). Biotinylated antibodies were...anti ZZQQhyFoxP3- eFlour450 (eBioscience) using the BrdU Flow Kit from...For Bcl-6 labeling, the eBioscienceFoxP3 Staining Kit was used as...

Effects of Exogenous Recombinant APC in Mouse Models of Ischemia Reperfusion Injury and of Atherosclerosis

Karin C. A. A. Wildhagen, …Gerry A. F. Nicolaes, PLOS ONE | 07/17/2014

… IL-6 levels per mg heart homogenate were determined with mouse IL-6 ELISA Ready-set-go (eBiosciences). … Blood and spleen were collected, processed and fluorescently stained with either antibody

mix 1 (CD3-FITC, CD4-PerCp, CD8-eFLUO450, CD25-APC, Foxp3-PE and B220-PE-Cy7) or antibody mix2 (CD11b-PE-Cy7, Ly6C-APC, Ly6G-PE, CD3-FITC, B220-APC-eFLUO780 and NK1.1-PerCp-Cy5.5) or isotype control IgG (all BD Biosciences or eBioscience).

GM-CSF–Licensed CD11b+ Lung Dendritic Cells Orchestrate Th2 Immunity to Blomia tropicalis Qian Zhou, … David M. Kemeny, J. Immunol., Jul 2014; 193: 496 - 509.

...CD11c PerCPCy5.5 or FITC (N418; eBioscience), Ly-6G allophycocyanin (1A8...Pharmingen), CD3 eFluor450 (17A2; eBioscience), I-A/I-E eFluor450 (M5/1114.15.2;eBioscience)