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作者：eBioscience官网 发布日期：2014-04-17 08:00
 eBioscience公司Luminex液相蛋白芯片检测试剂——ProcartaPlex可以为您的生物学研究提供各种多因子检测方案，包括细胞因子、生长因子、趋化因子和其他蛋白。在选择试剂盒及操作时会遇到很多问题，现将常见问题及答复汇总如下，供您参考： 如果不小心将整个试剂盒保存在-20°C. 这个试剂盒还能再用吗？ I accidentally stored the whole kit at -20°C. Can I use it anyway? A. 试剂盒中除微球外，其余都可以保存在-20oC。如果微球在0oC以下保存，会损伤微球使得微球不能再用了。 All the components included in the ProcartaPlex kit are viable except the bead mixture. Storage of the beads at temperatures below 0oC will damage the beads and render them unusable. A.不需要。 No, when combining a Multiplex kit with Simplex Bead Sets, the Basic Kit is not required. All the non target specific reagents which are included in the Basic kit are components of our Multiplex kits. Only when you are combining multiple Simplex kits (i.e. no Multiplex kit in the panel) will you need to also purchase the Basic Kit. A. 不能。基础试剂盒有种属特异性。不同种属的基础试剂盒之间不能交叉使用。 No, the Basic Kits are specific to the species listed, i.e. the Mouse Basic Kit can only be used with Mouse Simplex Bead Sets. The Mouse, Human, Canine, Porcine, Rat and NHP ProcartaPlex Basic Kits are not interchangeable A.可以的。只要确保同时检测的试剂盒的微球编号不重复，样本前期处理相同即可。 Yes, you can combine ProcartaPlex kits from different orders to run a bigger multiplex experiment. You will want to ensure that there is no overlap of bead regions between the kits you are combining. Please refer to the online ProcartaPlex Panel Configurator. A.不行。检测ProcartaPlex (Luminex) 可以使用Luminex 100/200, MAGPIX, FLEXMAP 3D. To analyze ProcartaPlex (Luminex) plates, you will need access to a Luminex machine such as a Luminex 100/200, MAGPIX, FLEXMAP 3D. A.推荐每种微球收集50-100个。 Please collect 50-100 beads per bead region as stated in the product manual. A. 可以的。 Yes, it is possible to reread a finished ProcartaPlex plate without a loss in the signal or the number of beads counted. Please note that the Luminex instrument adds additional liquid to the wells with each analysis. It is possible that the wells may become overfilled with fluid after the third analysis. Hence, we do not recommend reading the ProcartaPlex plates more than two times. A. 正常情况，我们建议立即上机检测；如果需要保存，建议在2-8oC保存。但是时间不能超过1天。 Ideally, we recommend that you analyze the samples immediately. If you do need to store your samples, overnight storage at 2-8°C shows no significant signal loss. We do not recommend storing the ProcartaPlex plate longer than 1 day. A.不可以。 No, we do not recommend centrifuging the magnetic beads for prolonged periods of time. A. 不推荐。建议每次试验使用新的标准品。 The recombinant standards included with the ProcartaPlex assay are one-time use standards. Once reconstituted for use, we do not recommend storing and reusing this recombinant standard. With each new experiment, we recommend reconstituting a fresh unused vial of recombinant standard protein. A.请在随货收到的说明书中找到。 This information can be found on the Certificate of Analysis provided with the ProcartaPlex assay. A. 为了获得更好的结果准确性，我们建议样本和标准曲线孔至少做复孔。 For better accuracy, we recommend that you run samples/standards as duplicates at a minimum. A. 按照说明书推荐，可以在加入标准品和样本后过夜反应。 As stated in the product manual, after the addition of the standards and samples, the plate can be stored overnight (Step 11.1.5 or 11.2.5). |
