简介：
| 磷脂酰肌醇3-激酶(PI3K)通路是调控细胞生长、增殖、代谢、存活和血管发生的主要通路之一。许多癌症研究表明，在癌症病人中，参与PI3K/Akt信号转导的蛋白较其他蛋白有更高的表达变化、突变和易位。在癌症患者中，该通路是最过度活跃的通路之一，成为治疗该疾病的明显靶点。Akt，也称为PKB，是一种丝氨酸/苏氨酸蛋白激酶，由PH结构域、激酶催化结构域和调控结构域构成。Akt有三种亚型：Akt1、Akt2和Akt3，在各种组织中有不同水平的表达。AKT除了作为PI3K信号通路通路中的主要组分，还在多种信号网络中(如细胞因子、NK-κB、GPCRs和整合素等)扮演重要作用。活化的PI3K产生第二信使3 ,4 , 5-三磷酸磷脂肌醇(PIP3)，后者与Akt和磷酸肌醇依赖激酶(PDK1)结合，改变Akt的蛋白结构，使之移位到细胞膜，导致Akt活化。Akt上的Thr308和Ser473的磷酸化可使之完全活化，下调PTEN、PPA2和mTOR。PI3K/PTEN/Akt以及mTOR已成为治疗癌症、糖尿病、神经失调等的靶点。 |
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超分辨率(STED)荧光显微术。A431细胞经血清饥饿12h后，加入10μg/ml Anti Akt pS473 monoclonal antibody (Cat No. 200-301-268)进行检测。可观察到磷酸化的AKT(红色)位于胞质(A)和细胞外周(D)。B和E显示的是随后加入Anti Alpha Tubulin (Rabbit)(Cat No. 600-401-880)进行染色。叠加图(C和F)显示p-AKT和微管共定位。高倍图像(D、E和F)显示p-AKT邻近于微管。 |
| 产品名称 |
货号 |
规格 |
应用 |
目录价(￥) |
| Anti-AKT pS473 (MOUSE) Monoclonal Antibody |
RK-200-301-268 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pS473 (MOUSE) Monoclonal AntibodyDyLight 488 Conjugated |
RK-200-341-268 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pS473 (MOUSE) Monoclonal AntibodyDyLight 549 Conjugated |
RK-200-342-268 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pS473 (MOUSE) Monoclonal AntibodyDyLight 649 Conjugated |
RK-200-343-268 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT (MOUSE) Monoclonal Antibody |
RK-200-301-401 |
100μg |
E, WB, IP |
4704.00 |
| Anti-AKT (RABBIT) Antibody |
RK-100-401-401 |
200μl |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT (SHEEP) Antibody |
RK-600-601-401 |
100μg |
E, WB, IP |
4704.00 |
| Anti-AKT pS473 (RABBIT) Antibody |
RK-600-401-268 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pT308 (MOUSE) Monoclonal Antibody |
RK-200-301-269 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-AKT pT308 (MOUSE) MonoclonalAntibody DyLight 488 Conjugated |
RK-200-341-269 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pT308 (MOUSE) Monoclonal Antibody DyLight 549 Conjugated |
RK-200-342-269 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pT308 (MOUSE) Monoclonal Antibody DyLight 649 Conjugated |
RK-200-343-269 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-AKT pT308 (RABBIT) Antibody |
RK-600-401-269 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-AKT2 (RABBIT) Antibody |
RK-600-401-425 |
100μg |
E, WB, IP |
4704.00 |
| Anti-ATM Protein Kinase pS1981 (MOUSE)Monoclonal Antibody |
RK-200-301-400 |
100μg |
E, IF, IHC, WB, IP |
6320.00 |
| Anti-ATM Protein Kinase S1981 (RABBIT) Antibody |
RK-600-401-398 |
100μg |
E, IF, WB, IP |
5344.00 |
| Anti-BAX (RABBIT) Monoclonal Antibody |
RK-200-C01-B34 |
100μl |
E, IHC, WB, IP |
4704.00 |
| Anti-Cyclin D1 (RABBIT) Antibody |
RK-100-401-153 |
100μl |
E, WB, IP |
4704.00 |
| Anti-GSK3 alpha (RABBIT) Antibody |
RK-600-401-444 |
100μg |
E, WB, IP |
4704.00 |
| Anti-GSK3 alpha pS21 (RABBIT) Antibody |
RK-600-401-429 |
100μg |
E, WB, IP |
4704.00 |
| Anti-GSK3 beta pS9 (RABBIT) Antibody |
RK-600-401-424 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-IKBα C-terminal (RABBIT) Antibody |
RK-100-4167C |
100μl |
E, WB, IP |
4704.00 |
| Anti-IKBβ (RABBIT) Antibody |
RK-100-4186 |
100μl |
E, WB, IP |
4704.00 |
| Anti-IKKα (RABBIT) Antibody |
RK-100-401-219 |
100μl |
E, WB, IP |
4704.00 |
| Anti-IKKβ (RABBIT) Antibody |
RK-100-401-220 |
100μl |
E, WB, IP |
4704.00 |
| Anti-IKKε pT501 (RABBIT) Antibody |
RK-600-401-267 |
100μg |
E, WB, IP |
4704.00 |
| Anti-MDM2 (RABBIT) Antibody |
RK-600-401-927 |
100μg |
E, WB, IP |
4704.00 |
| Anti-mTOR (RABBIT) Antibody |
RK-600-401-897 |
100μg |
E, WB, IP |
4704.00 |
| Anti-mTOR pS2448 (RABBIT)Antibody |
RK-600-401-422 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-NFKB cRel (RABBIT) Antibod |
RK-100-4166 |
100μl |
E, WB, IP, GS |
4704.00 |
| Anti-NFKB p50 (NFKB1) (RABBIT)Antibody |
RK-100-4164 |
100μl |
E, IHC, WB, IP, GS |
4704.00 |
| Anti-NFKB p52 (RABBIT) Antibody |
RK-100-4185 |
100μl |
E, WB, IP, GS |
4704.00 |
| Anti-NFKB p65 (Rel A) (RABBIT)Antibody |
RK-100-4165 |
100μl |
E, IHC, WB, IP, GS |
4704.00 |
| Anti-NFKB p65 (Rel A) N-TERMINAL SPECIFIC (RABBIT) Antibody |
RK-100-4165N |
100μl |
E, WB, IP |
4704.00 |
| Anti-NFKB p65 (Rel A) pS276 (RABBIT)Antibody |
RK-100-401-264 |
100μl |
E, WB |
4704.00 |
| Anti-NFKB p65 (Rel A) pS529 (RABBIT) Antibody |
RK-100-401-266 |
100μl |
E, WB, IP |
4704.00 |
| Anti-NFKB p65 (Rel A) pS536 (RABBIT)Antibody |
RK-600-401-265 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-NFKB p65 NLS specific (RABBIT)Antibody |
RK-600-401-271 |
100μg |
E, IF, IHC, WB, IP |
4704.00 |
| Anti-NFKB p105 (RABBIT) Antibody |
RK-100-4184 |
100μl |
E, WB |
4704.00 |
| Anti-p21/WAF1 (MOUSE) MonoclonalAntibody |
RK-200-301-171 |
100μg |
E, WB, IP |
4704.00 |
| Anti-p27 (RABBIT) Antibody |
RK-100-401-172 |
100μl |
E, WB |
4704.00 |
| Anti-p53 (MOUSE) MonoclonalAntibody |
RK-200-301-174 |
100μg |
ChIP, E, IF, IHC, WB, IP |
4704.00 |
| Anti-p70 S6 Kinase [RABBIT) Antibody |
RK-600-401-942 |
100μg |
E, WB, IP |
4704.00 |
| Anti-Pdcd4 (RABBIT) Antibody |
RK-600-401-965 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-Pdcd4 pS457 (MOUSE) MonoclonalAntibody |
RK-200-301-964 |
100μg |
E, IHC, WB, IP |
4704.00 |
| Anti-PDK1 (RABBIT) Antibody |
RK-600-401-411 |
100μg |
E, WB, IP |
4704.00 |
| Anti-PI3-kinase p110 δ (delta subunit)(RABBIT) Antibody |
RK-100-401-862 |
100μl |
E, WB, IP |
4704.00 |
| Anti-PTEN-P1 (RABBIT) Antibody |
RK-600-401-A90 |
100μg |
E, WB, IP |
4704.00 |
| Anti-PTEN-P1 pS29/pT32 (RABBIT) Antibody |
RK-600-401-859 |
100μg |
E, WB, IP |
4704.00 |
| E: ELISA |
IF: Immunofluorescence |
IHC: Immunohistochemistry |
IP: Immunoprecipitation |
| GS: Gel Shift |
WB: Western Blotting |
ChIP: Chromatin immunoprecipitaion |
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| Anti-AKT (RABBIT) Antibody (Cat No. 100-401-401) 染色新生大鼠心肌细胞的免疫荧光图片 |
Anti-AKT pS473 (MOUSE) Monoclonal Antibody (Cat No. 200-301-268)染色人前列腺组织的免疫组化图片 |
使用Anti-GSK3 alpha (RABBIT) Antibody (Cat No. 600-401-444)在HEK293细胞裂解物中检测到52 kDa的GSK3α |
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