eFluor家族新成员

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http://www.liankebio.com/article-information_Newsletter-1265.html

作者：eBioscience官网 发布日期：2013-12-09 11:00

PE-eFluor® 610更优替代PE-Texas Red®
PE-eFluor® 610 适用于蓝光、绿光(488-561 nm)激发，最大发射为607 nm， 与PE-Texas Red®使用相同的检测通道。

相比 PE-Texas Red® 有更高的亮度，与红光的补偿却小。

正常人外周血染色浅橙色：CD3 PE-Texas Red®橙色：CD3 PE-eFluor® 610，荧光强度更高
相同条件下，PE-efluor610与PerCP-eFluor710及PE-Cy7的补偿都小于PE-Texas Red及BD Horizon™PE-CF594

PE-eFluor® 610 荧光强度与 PE 和 PerCP-eFluor® 710非常接近

正常人外周血染色结果左：Anti-Human CD19 APC (cat. 17-0199) 和 Anti-Human CD185 PE (cat. 12-9185)中：Anti-Human CD19 APC (cat. 17-0199) 和 Anti-Human CD185 PE-eFluor® 610 (cat. 61-9185)右：Anti-Human CD19 APC (cat. 17-0199) 和 Anti-Human CD185 PerCP-eFluor® 710(cat. 46-9185).

PE-eFluor® 610 固定后稳定性

正常人外周血细胞 CD3 PE-eFluor 610 (clone UCHT1)染色。细胞洗涤后立刻上机（红色曲线）或者在IC fixation buffer中4°C暗处孵育24小时（蓝色曲线）、48小时（橙色曲线）、72小时（绿色曲线）后洗涤上机，结果保持一致。

目前已有以下抗体供您选择：

Name Clone Cat. No.
Anti-Human CD3 PE-eFluor® 610 UCHT1 85-61-0038
Anti-Mouse CD3e PE-eFluor® 610 145-2C11 85-61-0031
Anti-Mouse CD4 PE-eFluor® 610 RM4-5 85-61-0042
Anti-Human CD4 PE-eFluor® 610 RPA-T4 85-61-0049
Anti-Mouse CD8a PE-eFluor® 610 53-6.7 85-61-0081
Anti-Human CD8a PE-eFluor® 610 RPA-T8 85-61-0088
Anti-Human CD11c PE-eFluor® 610 3.9 85-61-0116
Anti-Human CD19 PE-eFluor® 610 HIB19 85-61-0199
Anti-Human/Non-Human Primate CD25 PE-eFluor® 610 CD25-4E3 85-61-0257
Anti-Mouse CD25 PE-eFluor® 610 PC61.5 85-61-0251
Anti-Human CD45 PE-eFluor® 610 HI30 85-61-0459
Anti-Human/Mouse CD45R (B220) PE-eFluor® 610 RA3-6B2 85-61-0452
Anti-Mouse CD49b (Integrin alpha 2) PE-eFluor® 610 DX5 85-61-5971
Anti-Human CD56 (NCAM) PE-eFluor® 610 CMSSB 85-61-0567
Anti-Human CD185 (CXCR5) PE-eFluor® 610 MU5UBEE 85-61-9185
Anti-Human CD279 (PD-1) PE-eFluor® 610 eBioJ105 (J105) 85-61-2799
Anti-Mouse EOMES PE-eFluor® 610 Dan11mag 85-61-4875
Anti-Human EOMES PE-eFluor® 610 WD1928 85-61-4877
Anti-Mouse F4/80 Antigen PE-eFluor® 610 BM8 85-61-4801
Anti-Mouse/Rat Foxp3 PE-eFluor® 610 FJK-16s 85-61-5773
Anti-Human Foxp3 PE-eFluor® 610 PCH101 85-61-4776
Golden Syrian Hamster IgG Isotype Control PE-eFluor® 610 n/a 85-61-4914
Armenian Hamster IgG Isotype Control PE-eFluor® 610 eBio299Arm 85-61-4888
Mouse IgG1 K Isotype Control PE-eFluor® 610 P3.6.2.8.1 85-61-4714
Rat IgG2a K Isotype Control PE-eFluor® 610 eBR2a 85-61-4321
Mouse IgG2b K Isotype Control PE-eFluor® 610 　/ 85-61-4732
Rat IgM Isotype Control PE-eFluor® 610 　/ 85-61-4341
Anti-Mouse/Rat IL-17A PE-eFluor® 610 eBio17B7 85-61-7177
Anti-Human Ki-67 PE-eFluor® 610 20Raj1 85-61-5699
Anti-Mouse/Rat Ki-67 PE-eFluor® 610 SolA15 85-61-5698
Anti-Mouse KLRG1 PE-eFluor® 610 2F1 85-61-5893