隔壁老王又又又发高分论文了，我酸了

• 论 文 内 容 ：

T细胞分泌生物活性胞外囊泡(EVs)，但CD4+ T细胞EVs的潜在生物学效应尚不清楚。本研究的主要目的是探讨CD4+ T细胞来源的EVs对B细胞应答的影响，并探讨其在抗原介导的体液免疫应答中的作用。在本研究中，CD4+ T细胞EVs从活化的CD4+中纯化体外培养的T细胞。经乙肝表面抗原免疫后接种乙肝疫苗后，CD4+ T细胞EVs处理的小鼠表现出更强的体液免疫反应，这是由血清中乙型肝炎表面抗体(HBsAb)水平升高和骨髓中浆细胞比例升高所显示的。

• 论 文 示 图：

作者应用ELISA、Western blot、FCM实验技术，通过实验分析得出：EVs参与CD4+ T细胞介导的B细胞应答。

Figure 1. Inhibition of EVs release impairs the function of CD4T cells in vitro. A) Bicinchoninic acid (BCA) protein assay of total protein (top) and immunoblot analysis of CD63 (bottom) in purified EVs from supernatants of CD4T cells treated with a control vehicle (dimethyl sulfoxide (DMSO)) or 10, 20, or 40 ×10−6 m GW4869 for 48 h. B) A schematic of the Transwell coculture model with B cells in the upper chamber and CD4T cells in the lower chamber of the well. A porous (0.4 m) membrane allows the transfer of EVs but precludes direct cell contact. C) Flow cytometry analysis of CD86 and MHCII expression on the surface of B cells. D) Total IgG levels in B cell supernatants were analyzed by enzyme-linked immunosorbent assay (ELISA). E) A schematic of the utilization of anti-CD63 microbeads to remove EVs in CD4T cell supernatant. F) The removal efficiency of CD63-positiveEVs in the supernatant was evaluated by western blotting. G) CD86 and MHCII expression on the surface of B cells cultured with different supernatants for 48 h was analyzed by flow cytometry (FCM). H) B cells were cultured with different supernatants for 72 h, and the total IgG in the supernatant was analyzed by ELISA. *P 0.05, **P 0.01, and ***P 0.001 (Student’s t-test). The data are from three independent experiments (A (top), C, D, F (left),G, and H; mean and s.e.m.) or are representative of three independent experiments (A (bottom) and F (right)).

• 作者单位：江苏大学医学院

• 论文期刊名：Advanced Science

• 影响因子：15.8

• 应用产品：Mouse IgG ELISA Kit

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