糖酵解实际上在几乎所有的原核细胞和真核细胞中都有发现。这一途径在肿瘤细胞中高度上调(瓦博格效应--Warburg effect)。参与糖酵解的酶是癌症治疗的潜在目标。BioVision提供实验测定糖酵解途径的各种代谢物,辅助因子和酶的一系列测定产品。
试剂盒特点:
? 非放射性,均质检测
? 特异性检测
? 方便:最小样本处理;快速方案(1-2小时)
? 性价比高:100次;高通量筛选兼容
? 经过验证:可用与哺乳动物组织,细胞和体液
靶点 |
货号 |
检测方法 |
检测极限 |
样本类型 |
代谢物 |
2-Phosphoglycerate |
93- K778 |
C/F |
20 pmol |
Cells, tissues |
Dihydroxyacetone Phosphate |
93- K673 |
F |
50 pmol |
Cells, tissues |
Glucose |
93- K606 |
C/F |
100 pmol |
Serum, cells, tissues, urine |
Glucose |
93- K686 |
C |
1000 pmol |
Serum, cells, tissues, food |
Glucose |
93- K688 |
F |
10 pmol |
Serum, cells, tissues, food |
Glucose-6-phosphate |
93- K657 |
C |
1000 pmol |
Serum, cells, tissues |
Glucose-6-phosphate |
93- K687 |
F |
5 pmol |
Serum, cells, tissues |
Phosphoenol Pyruvate |
93- K365 |
C/F |
100 pmol |
Cells, tissues |
Pyruvate |
93- K609 |
C/F |
100 pmol |
Cells, tissues |
|
酶 |
Aldolase |
93- K665 |
C |
100 mU |
Cells, tissues |
Enolase |
93- K691 |
C/F |
0.04 mU |
Cells, tissues |
GADPH |
93- K680 |
C |
10 mU |
Cells, tissues |
Hexokinase |
93- K769 |
F |
2 μU |
Serum, cells, tissues |
Hexokinase |
93- K713 |
C |
--- |
Potential Inhibitors |
Phosphofructokinase |
93- K776 |
C |
1 mU |
Cells, tissues |
Phosphoglucose isomerase |
93- K775 |
C |
0.1 mU |
Serum, cells, tissues |
Pyruvate kinase |
93- K709 |
C/F |
1000 pmol |
Cells, tissues |
Triose Phosphate Isomerase |
93- K670 |
C |
4 mU |
Cells, tissues |
|
辅酶 |
ADP |
93- K356 |
C |
1000 pmol |
Tissue, cells, PP |
ADP |
93- K355 |
C/F |
100 pmol |
Tissue, cells, PP |
ATP |
93- K354 |
C/F |
100 pmol |
Tissue, cells, PP |
ADP/ATP Ratio |
93- K255 |
L |
< 10 pmol |
Cells, PP |
NADH |
93- K338 |
F |
< 0.5 pmol |
Tissue, cells, PP |
NAD/NADH Ratio |
93- K337 |
C |
10 pmol |
Tissue, cells, serum, urine |
|