文章目录[隐藏]
类风湿性关节炎（RA）是一种自身免疫性疾病。早期的类风湿性关节炎一般首先影响到小关节，随着病情的发展，症状蔓延到手腕、膝盖、脚踝、肘部、臀部和肩膀，最终导致骨侵蚀和关节畸形。RA微环境往往伴随着高度炎症、内源性气体水平失衡和治疗不力的恶性循环。为了打破这个循环，重庆大学、成都中医药大学、重庆医科大学附属第一医院的Wenbo Geng、Jie Zhao 、Bailong Tao等团队开发了一种双气体介导的可注射水凝胶，用于调节RA的免疫微环境，同时释放治疗药物。该研究成果在Bioactive Materials（IF=18.9）在线发表了题目为“Regulation of rheumatoid arthritis microenvironment via a self-healing injectable hydrogel for improved inflammation elimination and bone repair”的研究论文，此论文使用了联科生物Mouse IL-6 ELISA Kit（货号：EK206）、Mouse TNF-α ELISA Kit（货号：EK282）和Mouse IL-10 ELISA Kit（货号：EK210）3种检测试剂盒。
本文使用的水凝胶（DNRS凝胶）可以通过消耗过量的一氧化氮（NO）和释放治疗性硫化氢（H2S）按需分解，导致内源性气体恢复、炎症缓解和巨噬细胞极化为M2型。此外，水凝胶还可以抑制破骨细胞生成，增强成骨能力。在胶原诱导的关节炎大鼠模型中，关节内注射含有甲氨蝶呤的水凝胶（MTX/DNRS凝胶）显著减轻了炎症和临床症状，并促进了骨侵蚀的修复。体内结果表明，MTX/DNRS凝胶恢复了微环境，提高了MTX的治疗效果。该研究为开发用于RA治疗的多功能智能递送平台提供了新的思路。
Schematic illustration of the fabrication of self-healing injectable hydrogel and the mechanism of bone protection by regulating the microenvironment of RA. DNRS gel depletes excessive NO and releases H2S in the presence of cysteine, and sustained release MTX.
类风湿性关节炎相关指标
| TNF-α | IL-6 | CRP |
| IgG | IgM | IL-1β |
| IL-10 | IFN-γ | IgA |
| IL-8 | IL-2 | IL-4 |
产品的部分引用文献
1.Li G, Liu S, Chen Y, et al. An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment[J]. Nature Communications, 2023, 14(1): 3159.
2.Wu H, Du X, Xu J, et al. Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer[J]. Acta Biomaterialia, 2023, 157: 551-565.
3.Hao Y, Li H, Ge X, et al. Tumor‐Selective Activation of Toll‐Like Receptor 7/8 Agonist Nano‐Immunomodulator Generates Safe Anti‐Tumor Immune Responses upon Systemic Administration[J]. Angewandte Chemie, 2022, 134(52): e202214992.
1.Wu H, Du X, Xu J, et al. Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer[J]. Acta Biomaterialia, 2023, 157: 551-565.
2.Liu A, Zhang M, Wu Y, et al. ASPS Exhibits Anti‐Rheumatic Effects by Reprogramming Gut Microbiota and Increasing Serum γ‐Glutamylcysteine Level[J]. Advanced Science, 2023, 10(3): 2205645.
3.Kang J Y, Xu M M, Sun Y, et al. Melatonin attenuates LPS-induced pyroptosis in acute lung injury by inhibiting NLRP3-GSDMD pathway via activating Nrf2/HO-1 signaling axis[J]. International Immunopharmacology, 2022, 109: 108782.
1.Zhao Y Z, Huang Z W, Zhai Y Y, et al. Polylysine-bilirubin conjugates maintain functional islets and promote M2 macrophage polarization[J]. Acta biomaterialia, 2021, 122: 172-185.
2.Wang Y M, Ji R, Chen W W, et al. Paclitaxel alleviated sepsis-induced acute lung injury by activating MUC1 and suppressing TLR-4/NF-κB pathway[J]. Drug Design, Development and Therapy, 2019: 3391-3404.
3.Chen Y, Wang Y F, Song S S, et al. Potential shared therapeutic and hepatotoxic mechanisms of Tripterygium wilfordii polyglycosides treating three kinds of autoimmune skin diseases by regulating IL-17 signaling pathway and Th17 cell differentiation[J]. Journal of Ethnopharmacology, 2022, 296: 115496.
参考文献
Geng W, Zhao J, Tao B, et al. Regulation of rheumatoid arthritis microenvironment via a self-healing injectable hydrogel for improved inflammation elimination and bone repair[J]. Bioactive Materials, 2024, 36: 287-300.
原文链接
https://www.sciencedirect.com/science/article/pii/S2452199X24000835?via%3Dihub
