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2023年9月18日来自中南大学湘雅二医院黎志宏教授团队在知名期刊MedComm（IF=9.9）发表了Integrative analysis of TROAP with molecular features, carcinogenesis, and related immune and pharmacogenomic characteristics in soft tissue sarcoma的文章，此论文使用了联科生物Cell Cycle Staining Kit（货号：CCS012）检测试剂盒进行了细胞周期的验证实验。
The Cell Cycle Staining Kit (MULTI SCIENCES) was used for flow cytometry detection. According to the operating manual, a proper amount of transfected SW982 cells were collected, and the supernatant was discarded by centrifugation. The cells were washed once with PBS, and the supernatant was discarded by centrifugation. After adding 1 mL DNA staining solution and 10 μL permeabilization solution, vortex for 5–10 s to mix, incubate at room temperature for 30 min in the dark. Finally, the samples were tested on a flow cytometer.
(E) The flow cytometric analysis results of the cell cycle for SW982 cells in NC and TROAP-siRNA groups (n = 3 for each group). The transfected SW982 cells were collected and tested according to the section Materials and Methods. NC, negative control; siRNA, small interfering RNA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant.
该团队揭示了营养素相关蛋白（TROAP）在软组织肉瘤（STS）中呈现出升高的趋势，并且其升高与STS的不良预后相关。此外，TROAP的降低与免疫细胞浸润增加、基质增强和免疫激活上升相关。同时，基于TROAP的基因组特征被验证为可靠地预测患者的预后、免疫治疗和药物反应。基于年龄、转移状态和一个STS个体的TROAP衍生风险评分构建的一个正态图可以用来量化STS的生存概率。
此外，体外实验表明TROAP在STS中过度表达，而TROAP的下调可影响STS细胞的增殖、迁移、转移和细胞周期。总之，TROAP在STS组织和细胞中表达升高，与STS的不良预后和恶性生物行为相关。它可能作为STS诊断和治疗的潜在预后生物标志物。
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参考文献：
Tu C, Liu B, Li C,et al. Integrative analysis of TROAP with molecular features, carcinogenesis, and related immune and pharmacogenomic characteristics in soft tissue sarcoma. MedComm (2020). 2023 Sep 18;4(5):e369.
原文链接：
https://onlinelibrary.wiley.com/doi/abs/10.1002/mco2.369
