接招:Novus外泌体研究总攻略

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http://www.liankebio.com/article-information_Newsletter-756.html

作者:Novus官网 发布日期:2016-09-13 10:00

接招:Novus外泌体研究总攻略外泌体是一种直径为30-100nm的纳米级脂质包裹体结构,内部包裹了蛋白、mRNA和microRNA等物质,包括肿瘤细胞在内的几乎所有类型的细胞,都可以产生并释放外泌体。外泌体由细胞分泌释放出来,在血液等体液内传播,最后又可被其他细胞吞噬,是细胞间通讯的重要介质。

外泌体目前被视为特异性分泌的膜泡,参与细胞间通讯,对外泌体的研究兴趣日益增长,从外泌体研究方向而言,干细胞、免疫、microRNA、靶向给药等都是热门研究领域,2013年诺贝尔生物/医学奖授予三位在细胞囊泡方面贡献突出的科学家,使外泌体的研究达到全新的高度,外泌体将在癌症的诊断及治疗等领域发挥重要作用。
外泌体产生过程的示意图:
Novus提供提供多种试剂、工具和方案用于分离、表征和分析外泌体RNA和蛋白质内含物,部分产品列举如下:
1.外泌体定量试剂盒
产品特点:适用不同样本类型;仅需100ul样本;采用夹心法ELISA技术。

产品原理图:部分产品列表:

目录号 产品名称 样本类型
H6-NBP2-49782 Overall Exosome Capture and Quantification 血浆
H6-NBP2-49783 Overall Exosome Capture and Quantification 尿液
H6-NBP2-49785 Overall Exosome Capture and Quantification 血清
H6-NBP2-49787 Overall Exosome Capture and Quantification 细胞培养物
H6-NBP2-49786 Tumor-derived Exosome Enrichment & Quantification 体液、细胞培养物

2.外泌体RNA提取试剂盒
产品特点:简单、高效提取外泌体总RNA;提取物适用于下游实验。

产品原理图:部分产品列表:

目录号 产品名称 样本类型
H6-NBP2-49789 Exosome RNA Extraction 纯化的外泌体
H6-NBP2-49784 Overall Exosome Capture and RNA Extraction 体液、细胞培养物
H6-NBP2-49788 Tumor-derived Exosome Capture & RNA Extraction 体液

3.外泌体标准品
产品特点:用于阳性质控;源于多种样本;经多种实验(FCM,WB,ELISA)反复验证。

产品原理图:

部分产品列表:

目录号 外泌体来源 目录号 外泌体来源
H6-NBP2-49838 Plasma (Human) H6-NBP2-49860 DAUDI Cells (Human)
H6-NBP2-49842 Saliva (Human) H6-NBP2-49854 HCT116 Cells (Human)
H6-NBP2-49827 Serum (Human) H6-NBP2-49864 K562 Cells (Human)
H6-NBP2-49840 Urine (Human) H6-NBP2-49847 MM1 Cells (Human)
H6-NBP2-49862 A549 Cells (Human) H6-NBP2-49856 PC3 Cells (Human)
H6-NBP2-49849 BLCL21 Cells (Human) H6-NBP2-49852 SK-N-SH Cells (Human)
H6-NBP2-49858 BPH-1 Cells (Human) H6-NBP2-49844 U87-MG Cells (Human)
H6-NBP2-49845 COLO1 Cells (Human) H6-NBP2-49866 B16F10 Cells (Mouse)

4.外泌体预吸附板
产品特点:预吸附外泌体相关的捕获抗体;仅需100ul样品;操作简单、即用型。

产品原理图:

部分产品列表:

目录号 产品名称 检测方法
H6-NBP2-49802 Overall Exosome Isolation from Biofluids Colorimetric
H6-NBP2-49803 Overall Exosome Isolation from Biofluids Luminometric
H6-NBP2-49804 Overall Exosome Isolation from Biofluids Fluorometric
H6-NBP2-49805 Overall Exosome Isolation from Serum Colorimetric
H6-NBP2-49806 Overall Exosome Isolation from Serum Luminometric
H6-NBP2-49807 Overall Exosome Isolation from Serum Fluorometric
H6-NBP2-49808 Overall Exosome Isolation from Saliva Colorimetric
H6-NBP2-49809 Overall Exosome Isolation from Saliva Luminometric
H6-NBP2-49810 Overall Exosome Isolation from Saliva Fluorimetric
H6-NBP2-49811 Overall Exosome Isolation from Cell Media Colorimetric
H6-NBP2-49812 Overall Exosome Isolation from Cell Media Luminometric
H6-NBP2-49813 Overall Exosome Isolation from Cell Media Fluorimetric
H6-NBP2-49814 Tumor-derived Exosome Isolation from Plasma Colorimetric
H6-NBP2-49815 Tumor-derived Exosome Isolation from Plasma Luminometric
H6-NBP2-49816 Tumor-derived Exosome Isolation from Plasma Fluorimetric
H6-NBP2-49800 Neural-derived Exosome Isolation from Plasma Colorimetric
H6-NBP2-49817 Neural-derived Exosome Isolation from Plasma Luminometric
H6-NBP2-49818 Neural-derived Exosome Isolation from Plasma Fluorimetric
H6-NBP2-49819 Glial-derived Exosome Isolation from Plasma Colorimetric
H6-NBP2-49820 Glial-derived Exosome Isolation from Plasma Luminometric
H6-NBP2-49821 Glial-derived Exosome Isolation from Plasma Fluorimetric
H6-NBP2-49822 Monocytes/Platelet-derived Exosome Isolation from Plasma Colorimetric
H6-NBP2-49823 Monocytes/Platelet-derived Exosome Isolation from Plasma Luminometric
H6-NBP2-49824 Monocytes/Platelet-derived Exosome Isolation from Plasma Fluorimetric

5.外泌体分离微球
产品特点:富集分离外泌体;分离后便于解离,与下游实验兼容;提供0.4um和1.0um两种大小的微球;使用简单、高效、快速。

产品原理图:部分产品列表:

目录号 产品名称 样本类型 微球大小
H6-NBP2-49826 Overall Exosome Isolation Cell media 0.4 µm
H6-NBP2-49828 Overall Exosome Isolation Cell media 1.0 µm
H6-NBP2-49829 Tumor-Derived Exosome Isolation Biofluids 0.4 µm
H6-NBP2-49830 Tumor-Derived Exosome Isolation Biofluids 1.0 µm
H6-NBP2-49835 Overall Exosome Isolation Biofluids 0.4 µm
H6-NBP2-49836 Overall Exosome Isolation Biofluids 1.0 µm
H6-NBP2-49831 Overall Exosome Isolation Mouse biofluids 0.4 µm
H6-NBP2-49832 Overall Exosome Isolation Mouse biofluids 1.0 µm
H6-NBP2-49833 Overall Exosome Isolation Mouse cell media 0.4 µm

6.外泌体标记抗体s
部分产品列表:

目录号 标记物 来源/类型 应用 交叉反应性
H6-NBP1-90201 Alix Rb/Poly WB, ICC/IF, IHC-P Hu, Mu, Rt
H6-MAB5248 CD24 Mu/Mono IHC-P Hu
H6-NBP2-42225 CD63 Mu/Mono WB, DB, EM, ELISA,more Hu
H6-MAB5417 CD63 Rt/Mono Flow, IHC-Fr Mu
H6-NBP2-36567 CD63 Rt/Mono WB, Flow Hu, Mu
H6-NB100-77913 CD63 Mu/Mono WB, Flow, ICC/IF, more Hu
H6-NB100-65805 CD81 Mu/Mono WB, ELISA, Flow, ICC/IF, more Hu, Gt, Pm
H6-MAB4865 CD81 Rt/Mono Flow, Mu
H6-NBP2-22187 CD9 Mu/Mono WB, ELISA, Flow, IHC-P Hu
H6-MAB5218 CD9 Rt/Mono Flow, IHC-Fr Mu
H6-NB100-77915 CD9 Mu/Mono Flow, ICC/IF Hu, Bv, Ca,
H6-NB200-112 TSG101 Mu/Mono WB, ELISA, Flow,more Hu, Mu, Rt,
H6-NBP1-80659 TSG101 Rb/Poly WB, ICC/IF, IHC-P Hu, Mu, Rt

更多外泌体研究产品,请关注:Nouvs外泌体研究产品???????联科生物代理截止时间:2017年6月30日