eBioscience质谱流式细胞术应用抗体

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http://www.liankebio.com/article-information_Newsletter-1199.html

作者:eBioscience官网 发布日期:2014-04-16 09:00

eBioscience质谱流式细胞术应用抗体
CyTOF®质谱流式细胞术——The Scientist杂志评选出的2011年十大创新技术。

CyTOF质谱流式细胞术,采用金属元素标记物(通常是金属元素标记的特异抗体或者染料)标记或识别细胞表面和内部的信号分子,然后用流式细胞原理分离单个细胞,再用感应耦合等离子质谱(ICP-MS)观察单个细胞的原子质量谱,最后将原子质量谱的数据转换为细胞表面和内部的信号分子数据,并通过专业分析软件对获得的数据进行分析,从而实现对细胞表型和信号网络的精细观察。

这项研究通过这个可以称为大量细胞计数法的方法,观察了人类骨髓产生的不同形态细胞中及表面的34种物质,不但能正确归类10多种不同类型的免疫细胞,还能观察到各类免疫细胞的内部变化,从而预知可能发生的变化。这将有助于更快更广泛的测量处方药对人体细胞的反应及功效,提前发现细胞病变,研发出针对个人的治疗药物。

多数商业和学术用途中已报道适用于CyTOF®技术的抗体,都可以在eBioscience找到:

Name Clone Application Cat. No.
Anti-Human CD3 Functional Grade Purified UCHT1 FC, FA 85-16-0038
Anti-Human CD3 Purified UCHT1 FC, IHC, FA 85-14-0038
Anti-Mouse CD3e Functional Grade Purified 145-2C11 FC, FA 85-16-0031
Anti-Mouse CD3e Purified 145-2C11 FC, IHC, FA, IP, WB 85-14-0031
Anti-Mouse CD4 Functional Grade Purified RM4-5 FC 85-16-0042
Anti-Mouse CD4 Purified RM4-5 FC, IHC, FA 85-14-0042
Anti-Human CD4 Functional Grade Purified RPA-T4 FC, FA 85-16-0049
Anti-Human CD4 Purified RPA-T4 FC, IHC, FA 85-14-0049
Anti-Mouse CD5 Purified 53-7.3 FC, IHC, IP 85-14-0051
Anti-Mouse CD8a Functional Grade Purified 53-6.7 FC, FA 85-16-0081
Anti-Mouse CD8a Purified 53-6.7 FC, IHC, IP 85-14-0081
Anti-Human CD8a Purified RPA-T8 FC, IHC 85-14-0088
Anti-Human CD9 Purified eBioSN4 (SN4 C3-3A2) FC, IHC, IP 85-14-0098
Anti-Human CD11b Purified ICRF44 FC, IHC, FA 85-14-0118
Anti-Mouse CD11b Functional Grade Purified M1/70 FC, FA 85-16-0112
Anti-Mouse CD11b Purified M1/70 FC, IHC, FA, IP 85-14-0112
Anti-Human CD11c Purified 3.9 FC, IHC 85-14-0116
Anti-Mouse CD11c Functional Grade Purified N418 FC 85-16-0114
Anti-Mouse CD11c Purified N418 FC, IHC, IP 85-14-0114
Anti-Human CD13 Purified WM-15 (WM15) FC, IHC 85-14-0138
Anti-Human CD19 Functional Grade Purified HIB19 FC, FA 85-16-0199
Anti-Human CD19 Purified HIB19 FC, IHC, FA 85-14-0199
Anti-Human CD20 Purified 2H7 FC, IHC 85-14-0209
Anti-Human CD27 Purified O323 FC 85-14-0279
Anti-Human CD28 Functional Grade Purified CD28.2 FC, FA 85-16-0289

参考文献:

Bendall, S.C., et al., Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.Science. 332(6030): p. 687-96.