CyTOF®质谱流式细胞术——The Scientist杂志评选出的2011年十大创新技术。
CyTOF质谱流式细胞术,采用金属元素标记物(通常是金属元素标记的特异抗体或者染料)标记或识别细胞表面和内部的信号分子,然后用流式细胞原理分离单个细胞,再用感应耦合等离子质谱(ICP-MS)观察单个细胞的原子质量谱,最后将原子质量谱的数据转换为细胞表面和内部的信号分子数据,并通过专业分析软件对获得的数据进行分析,从而实现对细胞表型和信号网络的精细观察。
这项研究通过这个可以称为大量细胞计数法的方法,观察了人类骨髓产生的不同形态细胞中及表面的34种物质,不但能正确归类10多种不同类型的免疫细胞,还能观察到各类免疫细胞的内部变化,从而预知可能发生的变化。这将有助于更快更广泛的测量处方药对人体细胞的反应及功效,提前发现细胞病变,研发出针对个人的治疗药物。
多数商业和学术用途中已报道适用于CyTOF®技术的抗体,都可以在eBioscience找到:
Name |
Clone |
Application |
Cat. No. |
Anti-Human CD3 Functional Grade Purified |
UCHT1 |
FC, FA |
85-16-0038 |
Anti-Human CD3 Purified |
UCHT1 |
FC, IHC, FA |
85-14-0038 |
Anti-Mouse CD3e Functional Grade Purified |
145-2C11 |
FC, FA |
85-16-0031 |
Anti-Mouse CD3e Purified |
145-2C11 |
FC, IHC, FA, IP, WB |
85-14-0031 |
Anti-Mouse CD4 Functional Grade Purified |
RM4-5 |
FC |
85-16-0042 |
Anti-Mouse CD4 Purified |
RM4-5 |
FC, IHC, FA |
85-14-0042 |
Anti-Human CD4 Functional Grade Purified |
RPA-T4 |
FC, FA |
85-16-0049 |
Anti-Human CD4 Purified |
RPA-T4 |
FC, IHC, FA |
85-14-0049 |
Anti-Mouse CD5 Purified |
53-7.3 |
FC, IHC, IP |
85-14-0051 |
Anti-Mouse CD8a Functional Grade Purified |
53-6.7 |
FC, FA |
85-16-0081 |
Anti-Mouse CD8a Purified |
53-6.7 |
FC, IHC, IP |
85-14-0081 |
Anti-Human CD8a Purified |
RPA-T8 |
FC, IHC |
85-14-0088 |
Anti-Human CD9 Purified |
eBioSN4 (SN4 C3-3A2) |
FC, IHC, IP |
85-14-0098 |
Anti-Human CD11b Purified |
ICRF44 |
FC, IHC, FA |
85-14-0118 |
Anti-Mouse CD11b Functional Grade Purified |
M1/70 |
FC, FA |
85-16-0112 |
Anti-Mouse CD11b Purified |
M1/70 |
FC, IHC, FA, IP |
85-14-0112 |
Anti-Human CD11c Purified |
3.9 |
FC, IHC |
85-14-0116 |
Anti-Mouse CD11c Functional Grade Purified |
N418 |
FC |
85-16-0114 |
Anti-Mouse CD11c Purified |
N418 |
FC, IHC, IP |
85-14-0114 |
Anti-Human CD13 Purified |
WM-15 (WM15) |
FC, IHC |
85-14-0138 |
Anti-Human CD19 Functional Grade Purified |
HIB19 |
FC, FA |
85-16-0199 |
Anti-Human CD19 Purified |
HIB19 |
FC, IHC, FA |
85-14-0199 |
Anti-Human CD20 Purified |
2H7 |
FC, IHC |
85-14-0209 |
Anti-Human CD27 Purified |
O323 |
FC |
85-14-0279 |
Anti-Human CD28 Functional Grade Purified |
CD28.2 |
FC, FA |
85-16-0289 |
参考文献:
Bendall, S.C., et al., Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.Science. 332(6030): p. 687-96.
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