Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells

  • 类型:
  • 作者:Gu Yuanxing, Zhou Yingshan, Shi Xinfeng, Xin Yongping, Shan Ying, Chen Cong, Cao Tong, Fang Weihuan, Li Xiaoliang
  • 期刊:ARCHIVES OF VIROLOGY
  • 阅读原文

Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.

文章引用产品列表