Preparation, structure characterization of carboxymethylated schisandra polysaccharides and their intervention in immunotoxicity to polychlorinated biphenyls

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  • 作者:Ting Zhao, Yuchao Guo, Siyu Yan, Ning Li, Hongchen Ji, Qinhan Hu, Min Zhang, Qian Li, Hong Gao, Liuqing Yang, Xiangyang Wu
  • 期刊:PROCESS BIOCHEMISTRY
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Carboxymethylation is a well-known modification process for polysaccharides. To evaluated structural features and biological availability of carboxymethyl, carboxymethylated schisandra polysaccharides (CSP) was synthesized using an optimized chloroacetic acid /NaOH method. Optimal modification parameters were reaction temperature of 62.67 ℃, reaction time of 4.27 h, and 0.83 g chloroacetic acid. The main fraction of CSP (CSPP) was obtained by purification using DEAE-52 column. Chemical analysis indicated that CSPP contained carboxymethyl groups with a Mw of 1.698 × 104 g/mol and degree of substitution of 0.88 and composed of mannose, glucose and galactose at ratio of 1: 44.8: 3.7. Moreover, CSPP have improved water solubility (12.56 ± 0.04 mg/mL) and existed as a slight extended flexible chain. CSPP exhibited higher immunomodulating activities to polychlorinated biphenyl-126 (PCB126) -exposure mice than unmodified polysaccharide, as shown by improving PCB 126-induced reduction of body weight gain and atrophy of the immune organ, diminishing PCB126-induced inhibition of cytokines production and reversing damage of spleen caused by oxidative stress in PCB126-treated mice. It could be concluded that introduction of carboxymethyl groups to polysaccharide can alter its physicochemical characteristic to enhance its immunoregulatory activity. This study suggested that CSPP potentially serve as an effective nutritional intervention agent to reverse the PCB126-induced immunosuppression.

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