Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses

There are few studies on seaweed polysaccharides with UV/H₂O₂ treatment, so the aim of this study was to evaluate the effects of UV/H₂O₂ treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF). Results showed that the contents of reducing sugar and sulfate in PSF with UV/H₂O₂ treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides. In addition, UV/H₂O₂ treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology. Besides, polysaccharides after UV/H₂O₂ treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells. Typically, PSF with UV/H₂O₂ treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α). Therefore, UV/H₂O₂ treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.

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