The present study is aiming at evaluating the hepatoprotective of Radix Bupleuri extracts (RBE) on the d-galactosamine/lipopolysaccharide (D-GalN/LPS) induced liver injury of hybrid grouper in vitro and in vivo. In vitro, RBE (0, 200, 400 and 800?μg/ml) was added to the hybrid grouper primary hepatocytes before (pretreatment) the incubation of the hepatocytes with D-GalN (20?mM) plus LPS (1?μg/ml) in the culture medium. RBE at concentrations of 200, 400 and 800?μg/ml significantly improved cell viability and inhibited the elevation of TNF-α, IL-1β and IL-6 and significantly down-regulated the caspase-3, caspase-9 and P53 mRNA levels. In vivo administration of RBE at the doses of 0, 200, 400, 800 and 1600?mg/kg in the diet for 8 weeks prior to D-GalN (500?mg/kg) and LPS (20?μg/kg) intoxication. The study indicated that the RBE not only ameliorated liver injury, as evidenced by well-preserved liver architecture, but also significantly increased hepatic antioxidant enzymes activities in the D-GalN/LPS-induced liver injury animal model. Further demonstrating the protective effects of the RBE, we found that pretreatment with the RBE up-regulated the expression of antioxidant genes (GPx and MnSOD), while down-regulated apoptosis-related genes (caspase-3, caspase-9 and P53), immune related genes (MHC2 and TLR3) and pro-inflammatory cytokines (TOR and IKKα) mRNA expression in the liver of hybrid grouper. In brief, the present study showed that RBE can protect hepatocyte injury induced by D-GalN/LPS through elevating antioxidant enzyme activity and suppressing apoptosis and immune inflammatory responses. The results support the use of RBE as a hepatoprotective in fish.
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Annexin V-APC/7-AAD Apoptosis Kit 细胞凋亡试剂盒
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¥780.00 – ¥1,860.00
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- AP105
- 凋亡试剂盒
Annexin V-APC/7-AAD Apoptosis Kit 细胞凋亡试剂盒
- ¥780.00 – ¥1,860.00
