Enhanced Therapeutic Potential of Nano-Curcumin against Subarachnoid Hemorrhage-Induced Blood-Brain Barrier Disruption through Inhibition of Inflammatory Response and Oxidative Stress

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  • 作者:Zhang, Z. Y., Jiang, M., Fang, J., Yang, M. F., Zhang, S., Yin, Y. X., Li, D. W., Mao, L. L., Fu, X. Y., Hou, Y. J., Fu, X. T., Fan, C. D. & Sun, B. L.
  • 期刊:Molecular neurobiology 54, 1-14 (2017)
  • 阅读原文

Curcumin and nano-curcumin both exhibit neuroprotective effects in early brain injury (EBI) after experimental subarachnoid hemorrhage (SAH). However, the mechanism that whether curcumin and its nanoparticles affect the blood-brain barrier (BBB) following SAH remains unclear. This study investigated the effect of curcumin and the poly(lactide-co-glycolide) (PLGA)-encapsulated curcumin nanoparticles (Cur-NPs) on BBB disruption and evaluated the possible mechanism underlying BBB dysfunction in EBI using the endovascular perforation rat SAH model. The results indicated that Cur-NPs showed enhanced therapeutic effects than that of curcumin in improving neurological function, reducing brain water content, and Evans blue dye extravasation after SAH. Mechanically, Cur-NPs attenuated BBB dysfunction after SAH by preventing the disruption of tight junction protein (ZO-1, occludin, and claudin-5). Cur-NPs also up-regulated glutamate transporter-1 and attenuated glutamate concentration of cerebrospinal fluid following SAH. Moreover, inhibition of inflammatory response and microglia activation both contributed to Cur-NPs' protective effects. Additionally, Cur-NPs markedly suppressed SAH-mediated oxidative stress and eventually reversed SAH-induced cell apoptosis in rats. Our findings revealed that the strategy of using Cur-NPs could be a promising way in improving neurological function in EBI after experimental rat SAH.

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