BioVision推荐：胆碱和乙酰胆碱检测试剂盒

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http://www.liankebio.com/article-information_Newsletter-449.html

作者：联科生物 发布日期：2015-12-17 00:00

Colorimetric/Fluorometric Kit（Catalog# K615-100；100 assays；-20℃贮存）? 产品描述
胆碱是一种强有机碱，是卵磷脂的组成成分，也存在于神经鞘磷脂之中，是机体可变甲基的一个来源而作用于合成甲基的产物，同时又是乙酰胆碱的前体。乙酰胆碱（AcetylCholine,ACh）是一种神经递质，能特异性的作用于各类胆碱受体，在组织内迅速被胆碱酯酶破坏，其作用广泛，选择性不高。在神经细胞中，乙酰胆碱是由胆碱和乙酰辅酶A在胆碱乙酰移位酶（胆碱乙酰化酶）的催化作用下合成的。胆碱和乙酰胆碱在许多生物学过程中发挥重要的作用。

BioVision的胆碱和乙酰胆碱定量试剂盒提供了一种简单、高灵敏的方法来定量检测各种生物学标本中胆碱和乙酰胆碱的含量。试剂盒基本原理如下：游离的胆碱可被甜菜碱醛氧化成甜菜碱（betaine），反应产物可与胆碱探针相互作用产生颜色或者荧光，从而可被普通酶标仪（570 nm）或者荧光计检测（Ex/Em = 535/587 nm）检测到。乙酰胆碱则可先使用乙酰胆碱酯酶转换成胆碱，然后再进行检测。
? 产品特点：
1. 简单、快速、方便，~40分钟

2. 试剂盒适用于多种类型标本：血液、细胞、培养基、发酵培养基等，且不需要对标本进行前期处理或纯化。

3. 可检测到10 pmol~5 nmol 的胆碱或乙酰胆碱。

目录号 产品名称 规格
K615-100 Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit 100 assays
K764-100 Acetylcholinesterase Activity Colorimetric Assay Kit 100 assays
K360-100 Beta-Secretase Activity Fluorometric Assay Kit 100 assays
K240-100 Calpain Activity Fluorometric Assay Kit 100 assays
K773-100 Catalase Activity Colorimetric/Fluorometric Assay Kit 100 assays
K615-100 Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit 100 assays
K253-25 DNA Damage Quantification Colorimetric Kit 25 assays
K264-100 Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit 100 assays
K261-100 Glutathione Colorimetric Assay Kit 100 assays
K251-100 Glutathione Fluorometric Assay Kit 100 assays
K762-100 Glutathione Peroxidase Activity Colorimetric Assay Kit 100 assays
K263-100 GST Colorimetric Activity Assay Kit 100 assays
K260-100 GST Fluorometric Activity Assay Kit 100 assays
K332-100 HAT Activity Colorimetric Assay Kit 100 assays
K331-100 HDAC Activity Colorimetric Assay Kit 100 assays
K330-100 HDAC Activity Fluorometric Assay Kit 100 assays
K340-100 HDAC Inhibitor Drug Screening Kit (Fluorometric) 100 assays
K342-25 HDAC1 Immunoprecipitation (IP) & Activity Assay Kit 25 assays
K341-25 HDAC2 Immunoprecipitation (IP) & Activity Assay Kit 25 assays
K343-100 HDAC3 Activity Fluorometric Assay Kit 100 assays
K344-25 HDAC3 Immunoprecipitation (IP) & Activity Assay Kit 25 assays
K363-100 HDAC3 Inhibitor Screening Kit (Fluorometric) 100 assays
K348-100 HDAC8 Activity Fluorometric Assay Kit 100 assays
K368-100 HDAC8 Inhibitor Screening Kit (Fluorometric) 100 assays
K244-100 Human Calpain 1 Inhibitor Screening Kit (Fluorometric) 100 assays
K339-100 InSitu HDAC Activity Fluorometric Assay Kit 100 assays
K796-100 Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) 100 assays
K795-100 Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit 100 assays
K797-100 Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) 100 assays
K822-100 N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit 100 assays
K772-100 Peroxidase Activity Colorimetric/Fluorometric Assay Kit 100 assays
K320-250 Senescence Detection Kit 250 assays
K325-100 SIRT1 Inhibitor/Activator Screening Kit (Fluorometric) 100 assays
K322-100 SIRT2 Inhibitor Screening Assay Kit (Fluorometric) 100 assays
K324-100 Sirtuin Activity Assay Kit (Fluorometric) 100 assays
K335-100 Superoxide Dismutase (SOD) Activity Assay Kit 100 assays
K366-100 TACE Inhibitor Screening Assay Kit ( Fluorometric) 100 assays
K763-100 Thioredoxin Reductase Activity Colorimetric Assay Kit 100 assays
K274-100 Total Antioxidant Capacity (TAC) Colorimetric Assay Kit 100 assays
K571-100 Transglutaminase Activity Assay Kit (Colorimetric) 100 assays
K710-100 Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit 100 assays
K685-100 Xanthine/Hypoxanthine Colorimetric/Fluorometric Assay Kit 100 assays
K4699-100 Apolipoprotein E4/Pan-ApoE (human) ELISA Kit 100 assays
K4787-100 Beta-NGF (human) ELISA Kit 100 assays
K4711-100 Neuregulin-1/NRG1-beta1 (human) ELISA Kit 100 assays
K4926-100 NQO1 (human intracellular) ELISA Kit 100 assays
K4771-100 RANTES (Mouse) ELISA Kit 100 assays
K4923-100 Sirtuin 1 (human intracellular) ELISA Kit 100 assays
K4924-100 Sirtuin 2 (human intracellular) ELISA Kit 100 assays
K4754-100 TACE (human) ELISA Kit 100 assays