http://www.liankebio.com/article-information_Newsletter-1115.html
作者:Novus官网 发布日期:2014-07-10 13:00
INSTA-Blot是Nouvs的特色产品,是一种即用型的WB膜,膜上已转印人、小鼠、大鼠等细胞或组织中提取的蛋白,经过严格的质控,性能稳定,使用方便,为特定蛋白在组织或细胞中的表达筛选提供最简单、最高效的解决方案。您无需进行样本制备、蛋白上样、SDS-PAGE凝胶电泳、转膜等操作,可直接进行抗体孵育。
INSTA_BLOT即用型Western blot操作步骤:
1:用100%的甲醇润湿膜,用TBST清洗两次,洗去多余的甲醇;
2:用5%脱脂奶粉(溶于TBST中)封闭30-60分钟,封闭抗体非特异结合位点;
3:孵育一抗,4度过夜,或室温1-2小时;
4:TBST洗涤5次,4度或室温孵育二抗60分钟;
5:TBST洗涤5次,显色。
Novus INSTA-Blot产品列表:
目录号 |
产品名称 |
规格 |
H6-NBP2-29911 |
INSTA-Blot Breast Tissue OncoPair |
1张膜 |
H6-NBP2-30125 |
INSTA-Blot Breast Tissue OncoPair |
1张膜 |
H6-NBP2-30126 |
INSTA-Blot Breast Tissue OncoPair |
1张膜 |
H6-NBP2-30127 |
INSTA-Blot Breast Tissue OncoPair |
1张膜 |
H6-NBP2-30333 |
INSTA-Blot Breast Tissue OncoPair |
5张膜 |
H6-NBP2-30128 |
INSTA-Blot Breast Tissue OncoPair [Azide Free] |
1张膜 |
H6-NBP2-30129 |
INSTA-Blot Colon Tissue OncoPair |
1张膜 |
H6-NBP2-30123 |
INSTA-Blot Female Lung Tissue OncoPair |
1张膜 |
H6-NBP2-30119 |
INSTA-Blot Human Brain |
1张膜 |
H6-NBP2-30114 |
INSTA-Blot Human Cell Lines |
1张膜 |
H6-NBP2-30113 |
INSTA-Blot Human Tissues |
1张膜 |
H6-NBP2-31378 |
INSTA-Blot Human Tissues |
1张膜 |
H6-NBP2-30122 |
INSTA-Blot Male Lung Tissue OncoPair |
1张膜 |
H6-NBP2-30120 |
INSTA-Blot Mouse Brain |
1张膜 |
H6-NBP2-30111 |
INSTA-Blot Mouse Tissues |
1张膜 |
H6-NBP2-30115 |
INSTA-Blot Multi-species Brain,Testis and Ovary |
1张膜 |
H6-NBP2-30117 |
INSTA-Blot Multi-species Liver, Lung, Spleen Tissues |
1张膜 |
H6-NBP2-30116 |
INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues |
1张膜 |
H6-NBP2-30118 |
INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas |
1张膜 |
H6-NBP2-30124 |
INSTA-Blot Ovary Tissue OncoPair |
1张膜 |
H6-NBP2-30121 |
INSTA-Blot Rat Brain |
1张膜 |
H6-NBP2-30112 |
INSTA-Blot Rat Tissues |
1张膜 |
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